anti cd62l pe cy7 mel 14  (Cytek Biosciences)

Journal: iScience

Article Title: Cyclophosphamide depletes tumor infiltrating T regulatory cells and combined with anti-PD-1 therapy improves survival in murine neuroblastoma

doi: 10.1016/j.isci.2022.104995

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Article Snippet: PE-Cy7 Anti-mouse CD62L (MEL-14) rat IgG2a, κ , BD Biosciences , Cat#560516; RRID: AB_1645257.

Techniques: Blocking Assay, Mutagenesis, Recombinant, Staining, Polymer, Plasmid Preparation, Proliferation Assay, Software, Multiplex Assay, Lysis

Journal: Cell Reports

Article Title: mRNA-encoded HIV-1 Env trimer ferritin nanoparticles induce monoclonal antibodies that neutralize heterologous HIV-1 isolates in mice

doi: 10.1016/j.celrep.2022.110514

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Article Snippet: PE-Cy7 Rat Anti-Mouse CD62L Clone MEL-14 , BD Biosciences , Cat#560516; RRID: AB_1645257.

Techniques: Marker, Recombinant, Plasmid Preparation, Transfection, Staining, Knock-In, Software

Journal: EMBO Molecular Medicine

Article Title: Low immunogenicity of malaria pre‐erythrocytic stages can be overcome by vaccination

doi: 10.15252/emmm.202013390

Figure Lengend Snippet: A Schematic overview of experimental design. C57BL/6 mice received 2 × 10 6 OT‐I cells alone or were additionally immunised with 10,000 γ‐radiation attenuated parasites intravenously. B Flow cytometry plots show the gating strategy for identifying K b ‐SIINFEKL + CD11a hi CD8 + T cells. C Temporal analysis of antigen‐specific CD8 + T‐cell responses. Peripheral blood was obtained on days 4, 7, 14, 21, 42 and 88 post‐immunisation with WT (triangles; n = 3–9), CSP SIINFEKL (orange squares; n = 4–8) or UIS4 SIINFEKL (blue circles; n = 4–10) sporozoites, or no parasites (diamonds; n = 2–5) and stained for K b ‐SIINFEKL + CD11a hi CD8 + T cells. Line graph shows mean values (± SEM) from representative experiments. (* P < 0.05; ** P < 0.01; *** P < 0.001; Welch’s t ‐test comparing CSP SIINFEKL and UIS4 SIINFEKL ). See Appendix Table for the number of mice used per timepoint and per group. See Appendix Table for exact P ‐values. D–H C57BL/6 mice ( n = 4 per group), which received 2 × 10 6 CFSE‐labelled OT‐I splenocytes, were immunised with 10,000 γ‐radiation attenuated WT, CSP SIINFEKL or UIS4 SIINFEKL sporozoites intravenously. 5 days later, mice were sacrificed, spleens harvested and splenocytes assessed for (D) CFSE dilution of CD8 + T cells, (E) CFSE dilution of antigen‐experienced K b ‐SIINFEKL + CD11a hi CD8 + T cells and stained ex vivo (F‐H) for effector CD8 + T‐cell surface markers. Shown are flow cytometry plots of K b ‐SIINFEKL co‐staining with markers of effector phenotypes: (F) CD11a hi , (G) CD62L lo and (H) CD49d hi .

Article Snippet: Anti‐mouse CD62L‐PE‐Cy7 (MEL‐14) , Thermo Fisher Scientific , Cat# 25‐0621‐82, RRID: AB_469633.

Techniques: Flow Cytometry, Staining, Ex Vivo

Journal: EMBO Molecular Medicine

Article Title: Low immunogenicity of malaria pre‐erythrocytic stages can be overcome by vaccination

doi: 10.15252/emmm.202013390

Figure Lengend Snippet: A–D C57BL/6 mice ( n = 4 per group), which received 2 × 10 6 CFSE‐labelled OT‐I splenocytes, were immunised with 10,000 γ‐radiation attenuated WT, CSP SIINFEKL or UIS4 SIINFEKL sporozoites intravenously. 5 days later, mice were sacrificed, spleens harvested and splenocytes assessed for (A) CFSE dilution of antigen‐experienced K b ‐SIINFEKL + CD8 + T cells and stained ex vivo for effector CD8 + T‐cell surface markers (B) CD11a hi , (C) CD62L lo and (D) CD49d hi . Bar charts show mean values (± SEM) from representative experiments (*** P < 0.001; one‐way ANOVA with Tukey’s multiple comparison test). See Appendix Table for exact P ‐values.

Article Snippet: Anti‐mouse CD62L‐PE‐Cy7 (MEL‐14) , Thermo Fisher Scientific , Cat# 25‐0621‐82, RRID: AB_469633.

Techniques: Staining, Ex Vivo, Hi-C

Journal: EMBO Molecular Medicine

Article Title: Low immunogenicity of malaria pre‐erythrocytic stages can be overcome by vaccination

doi: 10.15252/emmm.202013390

Figure Lengend Snippet: C57BL/6 mice received 2 × 10 6 OT‐I cells alone ( n = 2) or were additionally immunised with 10,000 γ‐radiation attenuated WT ( n = 3), CSP SIINFEKL ( n = 4) or UIS4 SIINFEKL ( n = 4) sporozoites intravenously. Spleens and livers were harvested either 14 or 42 days later, and proportions of CD8 + T cells expressing effector surface markers were quantified. Flow cytometry plots show representative percentages of CD8 + T cells co‐staining K b ‐SIINFEKL and markers of effector phenotype (CD11a hi , CD49d hi , CD62L lo , CD44 hi ).

Article Snippet: Anti‐mouse CD62L‐PE‐Cy7 (MEL‐14) , Thermo Fisher Scientific , Cat# 25‐0621‐82, RRID: AB_469633.

Techniques: Expressing, Flow Cytometry, Staining

Journal: EMBO Molecular Medicine

Article Title: Low immunogenicity of malaria pre‐erythrocytic stages can be overcome by vaccination

doi: 10.15252/emmm.202013390

Figure Lengend Snippet:

Article Snippet: Anti‐mouse CD62L‐PE‐Cy7 (MEL‐14) , Thermo Fisher Scientific , Cat# 25‐0621‐82, RRID: AB_469633.

Techniques: